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Flow Cytometry
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Cell Sorting Calculator
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Cell Sorting Calculator
Cell Sorting Calculator
1. Prep & Workflow
2. Sorter & Hardware
Cells Needed for Downstream Assay
Total Starting Cells (Pre-sort)
Target Cell Percentage
%
Post-sort Wash Steps
Assumes ~20% yield loss per post-sort wash/processing step.
Sort Instrument
Cytek Aurora CS Sorter
Sony SH800 Microfluidic Sorter
Nozzle / Chip Size
Collection Buffer Vol. (mL)
Primary Requirement
Total Cells Needed (Pre-sort)
1.57E+06
Calculated Breakdown
Target Cells Post-processing
Target Cells Sorted
Additional Total Cells Needed
Max Event Rate (events/sec)
Max Cell Conc.
Max Cell Size (μm)
Est. Sort Time
Est. Sort Vol. (mL)
Est. Total Vol. (mL)
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