Please fill out and submit the following forms when submitting a new project or using the Aurora CS or SH800:
- Project description form
- IBC/Biohazard form - if sample has an IBC protocol and/or is a biohazard (click on
- Aurora CS sort form
- SH800 sort form
These protocols have been used by researchers at IUB:
- Apoptosis detection – Annexin V-AF488 vs PI (Thermo Fisher)
- Cell Cycle – Propidium Iodide (UCSD) - add ethanol to cells
- Cell Proliferation (Thermo Fisher)
- Drosophila Embyro sorting
Useful guides:
- Antibody titration (Leinco)
- Sorting Guidelines (CCHMC RFCC)
Additional assays and sorting performed by researchers at IU Bloomington:
- Surface marker Staining and sorting (Stem & Adherent cells - BD Biosciences)
- Intracellular marker staining (BioLegend)
- Measurement of Green Fluorescent Protein (GFP) expression and sorting
- Single Cell Sorting
- Mitochondrial membrane potential (ThermoFisher)
- Viability assays (Live/Dead dyes - BioLegend)
- Bacterial viability & counting (Viability; Viability & counting - Thermo Fisher)
- Protocol for Sulfolobus fixation and cell cycle analysis available through IUCAT - Analysis of the Archaeal ESCRT Apparatus
Other assays available:
- BrdU incorporation
- Cell proliferation assays
- Intracellular Cytokine detection
- Multiparameter Immunofluorescence analysis and cell sorting
- Signal Transduction
- and much more!
Other resources for cell preparation and staining for flow cytometry:
- Current Protocols in Cytometry – (Facility has a copy on permanent loan from Libraries)
- Molecular Probes Handbook
- Cytometry Part A (Journal)
- UCSD Protocols Page
- BD Biosciences
- ThermoFisher protocols
- COPAS Select, Union Biometrica - search protocols
- Flow Cytometry with Plant Cells (Wiley, E-Book, IU Libraries)