Facility Services

Aurora CS (name TBD)

• 5 lasers (355nm, 407nm, 488nm, 561nm and 640nm)
• 3 scatter parameters
• Increased sensitivity for small particle detection
• 64 fluorescent detectors
• 4 nozzle sizes (70um, 85um, 100um, and 130um)
• bioBUBBLE containment system and temperature control
• Tube (1.5, 5, and 15ml) and multi-well plate sorting
• Aurora CS sort questionnaire

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SH800 (Persephone)

• 2 lasers (488nm and 561nm) w/ 6 fluorescent detectors
• 3 chip sizes (70um, 100um, and 130um) - chip selection guide
• bioBUBBLE containment system and temperature control
• Tube (1.5, 5, and 15ml) and multi-well plate sorting
• SH800 detector configuration and sort questionnaire

Please include the following text in any publications using the SH800: "This equipment was supported in part, with support from the Indiana Clinical and Translational Sciences Institute, funded in part by Grant Number UL1 TR002529 from the National Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award."

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CytoFLEX LX (Apollo)

• 4 lasers (405nm, 488nm, 561nm, 640nm) w/ 15 fluorescent detectors
• VSSC off the 405nm laser for small particle detection​
• Multi-well plate analysis
• Absolute counting
• CytoFLEX LX configuration

Please include the following text in any publications using the CytoFLEX LX: "This equipment was funded in part by the IU Office of Research through the Research Equipment Fund."

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MACSQuant VYB (Mercury)

• Mobile (on a cart); MACSQuant VYB configuration
• 3 lasers (405nm, 488nm, 561nm) w/ 8 fluorescent detectors
• FSC and SSC PMTs off the 561nm laser
• Single, multi-tube and 96-well plate sampling w/ chill racks for analysis at 4C
• Absolute counting
• Automated start-up and shut-down

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COPAS Select (Achelous)

• Large particle sorter with Advanced Acquisition Package (AAP) for increased sensitivity and faster analysis
• 1 laser (488nm) w/ 3 fluorescent detectors
• 1-way sorting into petri dish or multi-well plate
• Union Biometrica website

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Moxi Z, QuadroMACS, MiniMACS 

• Moxi Z - Cell Counter & Sizer (training available)
  • Reports both cell counts and concentrations of a cell population
  • Measures size distribution of the cell population
  • Size range 
    • M-type cartridge = 4-30-micron
    • S-type cartridge = 3-26-micron
• Quadro and mini-MACS (available for check-out)
  • Magnet-based gentle cell separation
  • Used in conjunction with Miltenyi Biotec's LD and LS columns

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